NAD-Dependent Protein Deacetylase Sirtuin-1 Mediated Mitophagy Regulates Early Brain Injury After Subarachnoid Hemorrhage.

Background: This study focuses on the role of SIRT1 in neuroinflammation caused by early brain injury (EBI) after subarachnoid hemorrhage (SAH), and explores its mechanism in mitophagy after SAH. Methods: C57BL/6J mice and primary microglia SAH in vivo and in vitro models were constructed to explore the expression level of SIRT1 in neuroinflammation after SAH. Subsequently, the brain edema content, blood-brain barrier (BBB) damage and neurological function scores of the mice were observed after using the SIRT1 inhibitor EX-527. q-PCR and Western blot were used to detect relevant genes and proteins, and enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-6, IL-1ß, and TNF-α inflammatory factors. Immunofluorescence staining was used to observe the positive level of SIRT1 and the degree of mitochondria-lysosome fusion, and transmission electron microscopy was used to observe mitochondrial damage and autophagosome levels. Results: In in vivo and in vitro experiments, we found that SIRT1 expression increased after SAH, and neurological deficits, brain edema, and blood-brain barrier damage after SAH were aggravated. Inhibiting SIRT1 further aggravates the aforementioned damage. In addition, EX-527 can also inhibit the level of mitophagy and aggravate neuroinflammation after SAH. Conclusion: Our results indicated that SIRT1 promotes mitophagy and alleviates neuroinflammation after SAH.

Ti-containing NPs in raw water and their removal with conventional treatments in four water treatment plants in Taiwan.

The ingestion of Ti-containing nanoparticles from drinking water has emerged as a concern in recent years. This study therefore aimed to characterize Ti-containing nanoparticles in water samples collected from four water treatment plants in Taiwan and to explore the challenges associated with measuring them at low levels using single particle-inductively coupled plasma mass spectrometry. Additionally, the study sought to identify the most effective processes for the removal of Ti-containing nanoparticles. For each water treatment plant, two water samples were collected from raw water, sedimentation effluent, filtration effluent, and finished water, respectively. Results revealed that Ti-containing nanoparticles in raw water, with levels at 8.69 µg/L and 296.8 × 103 particles/L, were removed by approximately 35% and 98%, respectively, in terms of mass concentration and particle number concentration, primarily through flocculation and sedimentation processes. The largest most frequent nanoparticle size in raw water (112.0 ± 2.8 nm) was effectively reduced to 62.0 ± 0.7 nm in finished water, while nanoparticles in the size range of 50-70 nm showed limited changes. Anthracite was identified as a necessary component in the filter beds to further improve removal efficiency at the filtration unit. Moreover, the most frequent sizes of Ti-containing nanoparticles were found to be influenced by salinity. Insights into the challenges associated with measuring low-level Ti-containing nanoparticles in aqueous samples provide valuable information for future research and management of water treatment processes, thereby safeguarding human health.

Surface reconstructed Fe@C1000 for enhanced Fenton-like catalysis: Sustainable ciprofloxacin degradation and toxicity reduction.

The Fe-based catalysts typically undergo severe problems such as deactivation and Fe sludge emission during the peroxymonosulfate (PMS) activation, which commonly leads to poor operation and secondary pollution. Herein, an S-doped Fe-based catalyst with a core-shell structure (Fe@CT, T = 1000°C) was synthesized, which can solve the above issues via the dynamic surface evolution during the reaction process. Specifically, the Fe0 on the surface of Fe@C1000 could be consumed rapidly, leaving numerous pores; the Fe3C from the core would subsequently migrate to the surface of Fe@C1000, replenishing the consumed active Fe species. The X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) analyses demonstrated that the reaction surface reconstructed during the PMS activation, which involved the FeIII in-situ reduction by S species as well as the depletion/replenishment of effective Fe species. The reconstructed Fe@C1000 achieved near-zero Fe sludge emission (from 0.59 to 0.08-0.23 mg L-1) during 5 cycles and enabled the dynamic evolution of dominant reactive oxygen species (ROS) from SO4·- to FeIVO, sustainably improving the oxidation capacity (80.0-92.5% in following four cycles) to ciprofloxacin (CIP) and reducing the toxicity of its intermediates. Additionally, the reconstructed Fe@C1000/PMS system exhibited robust resistance to complex water matrix. This study provides a theoretical guideline for exploring surface reconstruction on catalytic activity and broadens the application of Fe-based catalysts in the contaminants elimination.

Effects of biochar application on nitrogen transformation and N2O emission in a coastal saline-alkali soil.

The application of biochar can improve soil fertility and benefit sustainable agricultural development and carbon neutrality simultaneously. To better understand the effects of biochar addition on nitrogen transformation and N2O emission in a coastal saline-alkali soil and its potential mechanisms, we conducted a 60-day laboratory incubation experiment with six treatments, i.e., ammonium sulfate (N 150 mg·kg-1), ammonium sulfate + 0.4% (weight/weight) biochar, ammonium sulfate + 0.6% biochar, ammonium sulfate + 0.8% biochar, ammonium sulfate + 1.6% biochar, and ammonium sulfate + 0.2% biochar and 0.2% organic fertilizer (based on equivalent N basis). The results showed that soil nitrogen transformation was mainly affected by biochar addition at the early stage of incubation. Biochar addition significantly increased the contents of nitrate and ammonium. Biochar addition significantly increased soil net nitrification rate, but the magnitude of such increases decreased with increasing biochar addition level. Similar temporal change patterns of N2O emissions were observed in all treatments, and the N2O emissions mainly occurred in the first 30 days of incubation. Compared with the CK, biochar addition significantly reduced the cumulative N2O emission, and the decrement increased with increasing biochar addition levels. In conclusion, the effects of biochar and nitrogen fertilizer addition on soil nitrogen transformation and N2O emission varied with the application rate. Biochar addition with a rate of 0.8% (W/W) increased soil inorganic nitrogen content and decreased soil N2O emission. It could provide theoretical basis and reference for the formulation of reasonable plans for the improvement and utilization of biochar in coastal saline-alkali soil.

The Role of NCS1 in Immunotherapy and Prognosis of Human Cancer.

The Neural Calcium Sensor1 (NCS1) is a crucial protein that binds to Ca2+ and is believed to play a role in regulating tumor invasion and cell proliferation. However, the role of NCS1 in immune infiltration and cancer prognosis is still unknown. Our study aimed to explore the expression profile, immune infiltration pattern, prognostic value, biological function, and potential compounds targeting NCS1 using public databases. High expression of NCS1 was detected by immune histochemical staining in LIHC (Liver hepatocellular carcinoma), BRCA (Breast invasive carcinoma), KIRC (Kidney renal clear cell carcinoma), and SKCM (Skin Cutaneous Melanoma). The expression of NCS1 in cancer was determined by TCGA (The Cancer Genome Atlas Program), GTEx (The Genotype-Tissue Expression), the Kaplan-Meier plotter, GEO (Gene Expression Omnibus), GEPIA2.0 (Gene Expression Profiling Interactive Analysis 2.0), HPA (The Human Protein Atlas), UALCAN, TIMER2.0, TISIDB, Metascape, Drugbank, chEMBL, and ICSDB databases. NCS1 has genomic mutations as well as aberrant DNA methylation in multiple cancers compared to normal tissues. Also, NCS1 was significantly different in the immune microenvironment, tumor mutational burden (TMB), microsatellite instability (MSI), and immune infiltrate-associated cells in different cancers, which could be used for the typing of immune and molecular subtypes of cancer and the presence of immune checkpoint resistance in several cancers. Univariate regression analysis, multivariate regression analysis, and gene enrichment analysis to construct prognostic models revealed that NCS1 is involved in immune regulation and can be used as a prognostic biomarker for SKCM, LIHC, BRCA, COAD, and KIRC. These results provide clues from a bioinformatic perspective and highlight the importance of NCS1 in a variety of cancers.

[Spatial Distribution of Aerobic Bacteria, Sources and Risks of Nitrogen and Phosphorus in Taihu Lake Sediments].

The release of nitrogen and phosphorus from sediments into lake water will exacerbate the eutrophication of lakes and endanger ecological safety and human health. Microorganisms are indispensable in nitrogen and phosphorus conversion, and accurate analysis of the distribution characteristics and sources of nitrogen and phosphorus in sediments as well as their relationship with microorganisms is an important prerequisite for lake eutrophication control. Taking Taihu Lake as the study area, 30 surface sediment samples were collected, and the grain size, pH, organic matter (OM), dissolved organic carbon (DOC), total phosphorus (TP), total nitrogen (TN), nitrate nitrogen (NO3--N), and dissolved organic nitrogen (DON) along with some other index contents were measured and analyzed; accordingly, spatial distribution characteristics were analyzed. While using nutrient agar (NA), the number of aerobic bacteria (AB) was determined by plate counting in the medium. Combined with principal component analysis (PCA) and Pearson correlation analysis, the spatial distribution characteristics and sources of sediments and AB in Taihu Lake were explored. The characteristics of sediment pollution in Taihu Lake were studied using the comprehensive pollution index and the organic pollution index methods. The results revealed that the average sediment indicators of the surface layer of Taihu Lake were as follows:AB was 9.25×104 CFU·g-1, average particle size (MZ) was 17.59 µm, pH was 7.62, ω(OM) was 15.05 g·kg-1, ω(DOC) was 71.60 mg·kg-1, ω(TP) was 598.13 mg·kg-1, ω(TN) was 1113.92 mg·kg-1, ω(NO3--N) was 3.22 mg·kg-1, and ω(DON) was 22.60 mg·kg-1. The comprehensive pollution index (FF) showed that 13% of the Taihu Lake was moderately polluted, while 87% was heavily polluted. Excluding the area in the center of the lake, the southern lake area, and some lakes in the western part of the East Taihu Lake, TN in the rest of the area was moderately and severely polluted. In addition to the heavy pollution of Zhushan Bay, the TP in Taihu Lake was generally at light and moderate pollution. The organic pollution index (OI) showed that the organic pollution of the sediments of Taihu Lake was relatively light, majorly caused by organic nitrogen (ON) pollution. DOC, DON, TN, and OM in Taihu Lake were primarily derived from the influence of aquatic plants, and TP And AB were primarily derived from the influence of the external input of rivers. This research will provide theoretical support for lake eutrophication treatment and also provide new ideas for further analysis of AB to remove nitrogen and phosphorus pollution from sediments.

Clinical Efficacy of Dapagliflozin in the Treatment of Patients with Diabetic Nephropathy and Its Effect on Proteinuria Level.

Objective: This study aimed to analyze the clinical efficacy of dapagliflozin in the treatment of diabetic kidney disease and its impact on proteinuria levels in patients. Methods: Retrospective analysis of medical records of 176 patients with diabetic kidney disease treated at our hospital from January 2020 to January 2022. According to the different treatment methods, the patients were divided into a control group (n=88) receiving enalapril maleate treatment and an observation group (n=88) receiving dapagliflozin treatment. The clinical treatment effects, blood glucose levels, renal function indicators, inflammation factor indicators, and adverse reactions were compared between the two groups. Results: The total effective rate of treatment (97.73%) in the observation group was significantly higher than that (79.55%) in the control group (P<0.05). After treatment, the FPG, 2hPG, and HbAlc levels in the observation group were significantly lower than those in the control group (P<0.05). After treatment, the Scr, BUN, UmAlb, UAER, UACR, and 24-hour urine protein quantitative levels in the observation group were significantly lower than those in the control group (P<0.05). After treatment, the hs-CRP, IL-1ß, and TNF-α levels in the observation group were significantly lower than those in the control group (P<0.05). The incidence of adverse reactions in the observation group significantly lower than the control group (P<0.05). Conclusion: Compared with enalapril maleate alone, the combined application of dapagliflozin in the treatment of diabetic kidney disease has more significant clinical efficacy. It can further control patients' blood sugar, reduce their body's inflammatory response, alleviate or eliminate their proteinuria symptoms, promote the recovery of their renal function, and enhance the safety of their treatment to a certain extent, which helps to further improve the clinical treatment effect of patients.

Mechanically conditioned cell sheets cultured on thermo-responsive surfaces promote bone regeneration.

Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades. However, efficient harvest and handling of cell sheets remain challenging, including insufficient extracellular matrix content and poor mechanical strength. Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types. However, currently, there are no effective ways to apply mechanical loading to cell sheets. In this study, we prepared thermo-responsive elastomer substrates by grafting poly(N-isopropyl acrylamide) (PNIPAAm) to poly(dimethylsiloxane) (PDMS) surfaces. The effect of PNIPAAm grafting yields on cell behaviours was investigated to optimize surfaces suitable for cell sheet culturing and harvesting. Subsequently, MC3T3-E1 cells were cultured on the PDMS-g-PNIPAAm substrates under mechanical stimulation by cyclically stretching the substrates. Upon maturation, the cell sheets were harvested by lowering the temperature. We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning. Reverse transcription quantitative polymerase chain reaction and Western blot analyses further confirmed that the expression of osteogenic-specific genes and major matrix components were up-regulated. After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering.

Catalytic Ozonation of Polluter Benzene from -20 to >50 °C with High Conversion Efficiency and Selectivity on Mullite YMn2O5.

Catalytic decomposition of aromatic polluters at room temperature represents a green route for air purification but is currently challenged by the difficulty of generating reactive oxygen species (ROS) on catalysts. Herein, we develop a mullite catalyst YMn2O5 (YMO) with dual active sites of Mn3+ and Mn4+ and use ozone to produce a highly reactive O* upon YMO. Such a strong oxidant species on YMO shows complete removal of benzene from -20 to >50 °C with a high COx selectivity (>90%) through the generated reactive species O* on the catalyst surface (60 000 mL g-1 h-1). Although the accumulation of water and intermediates gradually lowers the reaction rate after 8 h at 25 °C, a simple treatment by ozone purging or drying in the ambient environment regenerates the catalyst. Importantly, when the temperature increases to 50 °C, the catalytic performance remains 100% conversion without any degradation for 30 h. Experiments and theoretical calculations show that such a superior performance stems from the unique coordination environment, which ensures high generation of ROS and adsorption of aromatics. Mullite's catalytic ozonation degradation of total volatile organic compounds (TVOC) is applied in a home-developed air cleaner, resulting in high efficiency of benzene removal. This work provides insights into the design of catalysts to decompose highly stable organic polluters.

Spontaneous FeIII/FeII redox cycling in single-atom catalysts: Conjugation effect and electron delocalization.

The mechanism of spontaneous FeIII/FeII redox cycling in iron-centered single-atom catalysts (I-SACs) is often overlooked. Consequently, pathways for continuous SO4 ·-/HO⋅ generation during peroxymonosulfate (PMS) activation by I-SACs remain unclear. Herein, the evolution of the iron center and ligand in I-SACs was comprehensively investigated. I-SACs could be considered as a coordination complex created by iron and a heteroatom N-doped carbonaceous ligand. The ligand-field theory could well explain the electronic behavior of the complex, whereby electrons delocalized by the conjugation effect of the ligand were confirmed to be responsible for the FeIII/FeII redox cycle. The possible pyridinic ligand in I-SACs was demonstrably weaker than the pyrrolic ligand in FeIII reduction due to its shielding effect on delocalized π orbitals by local lone-pair electrons. The results of this study significantly advance our understanding of the mechanism of spontaneous FeIII/FeII redox cycling and radical generation pathways in the I-SACs/PMS process.

Process analysis of asymmetric interaction between copper and atrazine in a system of macrophytes.

To clarify the mutual influence and inner processes between heavy-metal and pesticide pollutants, single copper and atrazine as well as binary mixtures were spiked in a system of aquatic Acorus tatarinowi Schott. The results show that: the total copper amount in roots was 23.31 and 41.46 times as much as those in leaves in single and co-contaminated copper pollution. In the solution, the copper removal reached equilibrium in 3 days. Atrazine raised plant-mediated copper removal by 20.69 % by calculating mass balance, and the increase in pH value and organic matter and the decrease of nitrate in solutions were key factors driving it. Correlation analysis demonstrated that the pH increase was mainly caused by the decline of nitrate and increases in organic matter in the solution. Hydroxyl units on the surface of organic matter in solutions provided binding sites for Cu2+, which was demonstrated by CO and OH peak position alterations in Fourier Transform Infrared Spectrometer. In turn, the root contained 2.56 and 2.04 times as much as atrazine in leaves in single and co-contaminated atrazine treatments. In the solution, atrazine removal became stable after 7 days. Cu2+ inhibited the total accumulation of atrazine in plants by 12.5 %. Copper-induced biological phenol-like components in solution decreased the total atrazine accumulation in A. tatatinowii.

Effects of Ag nanoparticles on plant growth, Ag bioaccumulation, and antioxidant enzyme activities in Phragmites australis as influenced by an arbuscular mycorrhizal fungus.

Ag nanoparticles (AgNPs) are considered an emerging contaminant in recent years, and their harmful effects on plants pose new concerns, especially in coexistence with soil microorganisms. Arbuscular mycorrhizal fungi (AMF), as mutualistic fungi with most terrestrial plants, may contribute to alleviating nanotoxicity in plants. Herein, AgNP toxicity of different concentrations (1, 5, 10, 50, 100 mg/kg) on reed (Phragmites australis (Cav.) Trin. ex Steudel) as influenced by mycorrhizal inoculation with Funneliformis mosseae was investigated. The results revealed that concentration is the main factor influencing the AgNP phytotoxicity; AgNP dose had biphasic effects on AMF colonization, plant biomass, and antioxidant enzyme activities. Thereinto, different antioxidant enzymes had different tolerances to AgNP stress, and the turning point of their activities was respectively the following: POD-5 mg/kg < SOD-10 mg/kg < CAT-50 mg/kg. The growth configuration (root:shoot ratio) of Phragmites australis increased firstly and then decreased to cope with the increasing AgNP concentration. Additionally, the Ag accumulation and translocation of AgNP-exposed plants were relatively lower than that of equivalent Ag+-exposed plants. However, AMF inoculation improved plant antioxidant capability and biomass growth in response to AgNP-induced toxicity. Meanwhile, AMF effectively regulated the root:shoot ratio to accommodate AgNP stress. The linear model fittings and heat maps showed that the mycorrhizal plants exhibited a higher Ag accumulative rate and root partitioning (Ag organ distribution: root > stem > leaf) than the non-inoculated plants. Overall, our results demonstrated that AMF could diminish the negative effects induced by AgNPs and promote Ag immobilization in plant roots so as to alleviate AgNP-posed environmental risks.

Molecule characterization of chemosensory and metabolism-related genes in the proboscis of Athetis lepigone.

Introduction: The moth species Athetis lepigone (Möschler) (Lepidoptera: Noctuidae), which has recently been identified as a pest of summer maize (Zea mays L.) in China, has demonstrated a rapid proliferation with in the Huang-Huai-Hai Plain region since its initial discovery in Hebei Province in 2005. It has become a prevalent pest of corn crops, and its ability to adapt quickly to its surroundings is currently being investigated. One of the key characteristics of its siphoning mouthparts is not only the feeding apparatus itself but also the chemosensory organs that enable the detection of chemical signals from the surrounding environment. However, there is a lack of comprehensive research on the genes responsible for chemosensory and metabolic mechanisms in the proboscises of male and female A. lepigone adults. Methods: In this study, we utilized transcriptome analysis to identify a total of fifty chemosensory genes from six distinct families, including 19 odorant-binding proteins (OBPs), 22 chemosensory proteins (CSPs), one co-receptor (Orco), six odorant receptors (ORs), four ionotropic receptors (IRs), and two sensory neuron membrane proteins (SNMPs) in the proboscis. Notably, seven OBPs, two CSPs, and one OR were discovered for the first time. Additionally, fourteen genes related to metabolism, including cytochrome P450 (CYPs) and carboxylesterases (CXEs), were also identified. Furthermore, a qualitative analysis was conducted on the relative transcript levels of eight related genes. The expression of 21 annotated chemosensory and metabolic genes was compared between A. lepigone adults and larvae using qRT-PCR, revealing tissue specificity. The majority of genes exhibited predominant expression in the antennae and proboscis during the adult stage, while showing slight expression in the combination of sixth-instar larval head oral appendages (maxilla, labium, and antenna) and pheromone gland-ovipositors of female adults. Results/discussion: Our study points to a new pest control strategies that these newly discovered genes have the potential to serve as targets for enhancing future pest control, including mating disruption and the use of food attractants. And it would be advantageous to ascertain the distribution of chemosensory gene expression and gain insights into the functionalities of these genes, thereby establishing a novel theoretical framework for the advancement of eco-friendly pesticides and efficient pest management strategies in the future.

The mechanism of arbuscular mycorrhizal enhancing cadmium uptake in Phragmites australis depends on the phosphorus concentration.

Arbuscular mycorrhizal fungi (AMF) is a vital strategy to enhance the phytoremediation of cadmium (Cd) pollution. However, the function of AMF was influenced by phosphorus (P) concentration. To reveal the effect of AMF on the Cd accumulation of host plants under different P concentrations and how the AMF and P interact, this study comparatively analyzed the regulatory effects of AMF on the Cd response, extraction, and transportation processes of Phragmites australis (P. australis) under different P levels, and explored its physiological, biochemical and molecular biological mechanisms. The study showed that AMF could induce different growth allocation strategies in response to Cd stress. Moreover, AMF promoted plant Cd tolerance and detoxification by enhancing P uptake, Cd passivation, Cd retention in the cell wall, and functional group modulation. Under P starvation treatments, AMF promoted Cd uptake by inducing Cd to enter the iron pathway, increased the transport coefficient by 493.39%, and retained Cd in stems. However, these effects disappeared following the addition of P. Additionally, AMF up-regulated the expression of ZIP, ZIP, and NRAMP genes to promote cadmium uptake at low, medium, and high phosphorus levels, respectively. Thus, the Cd response mechanism of the AMF-P. australis symbiotic system was P dose-dependent.

[Litter storage and water-holding capacity of typical forests in mountainous area of Southwest China]. / è¥¿å—å±±åŒºå ¸åž‹æ£®æž—æž¯è½ç‰©å‚¨é‡åŠæŒæ°´èƒ½åŠ›.

Current studies on water conservation capacity of litter in the mountainous area of Southwest China (MASC) mainly focus on local scale. Such results are difficult to evaluate the storage and water-holding capacity of litter in the whole MASC. In this study, the results of site-scale research in the MASC from 2004 to 2021 were collated (a total of 16 research sites and 70 data), as well as the storage and water-holding characteristics of litters of three typical forests in the MASC were compared and analyzed. The results showed that the water-holding processes of litter in coniferous forest, broadleaved forest and mixed forest were similar, which could be divided into three stages: rapid water absorption, gradual slowing, and stable. The absorption rate and duration of different forests were different in each stage. The broadleaved forest had the fastest water absorption rate, while coniferous forest had the slowest with the longest duration to reach stability. There was no significant difference in litter storage among diffe-rent forest types. The total litter storage of coniferous forest, broadleaved forest and mixed forest ranged from 8.26 to 8.82 t·hm-2. The significant spatial variations of litter storage in semi-decomposed layer resulted in that of total litter storage. The total maximum water-holding capacity of litters of the three forests ranged from 17.85 t·hm-2 to 19.87 t·hm-2, and the maximum water-holding rate of litter ranged from 200.6% to 228.0%. There was a positive correlation between the maximum water-holding capacity and litter storage in different forests. The total effective retention capacity of three forest litters ranged from 11.66 to 12.29 t·hm-2, while the total effective retention rate of three forests ranged from 128.1% to 145.2%. There were no significant differences in litter storage and water holding capacity among three forest types with two decomposition degrees in MASC.

How a functional soil animal-earthworm affect arbuscular mycorrhizae-assisted phytoremediation in metals contaminated soil?

Phytoremediation is a promising and sustainable technology to remediate the risk of heavy metals (HMs) contaminated soils, however, this way is limited to some factors contributing to slow plant growth and low remediation efficiency. As soil beneficial microbe, arbuscular mycorrhizal fungi (AMF) assisted phytoremediation is an environment-friendly and high-efficiency bioremediation technology. However, AMF-symbiotic formation and their functional expression responsible for HMs-polluted remediation are significantly influenced by edaphic fauna. Earthworms as common soil fauna, may have various effects on formation of AMF symbiosis, and exhibit synergy with AMF for the combined remediation of HMs-contaminated soils. For now, AMF-assisted phytoremediation incorporating earthworm coexistence is scarcely reported. Therefore, the main focus of this review is to discuss the AMF effects under earthworm coexistence. Effects of AMF-symbiotic formation influenced by earthworms are fully reviewed. Moreover, underlying mechanisms and synergy of the two in HMs remediation, soil improvement, and plant growth were comprehensively elucidated. Phenomenon of "functional synergism" between earthworms and AMF may be a significant mechanism for HMs phytoremediation. Finally, this review analyses shortcomings and prescriptions in the practical application of the technology and provides new insights into AMF- earthworms synergistic remediation of HMs-contaminated soils.

Partial Fibular Head Osteotomy is an Alternative Option in Treatment of Posterolateral Tibial Plateau Fractures: A Retrospective Analysis.

Objective: This study aimed to evaluate the short-term effects of partial fibular head osteotomy for treating posterolateral tibial plateau fractures. Methods: A retrospective analysis was performed on 25 patients with posterolateral tibial plateau fractures who were treated using a partial fibular head osteotomy approach. Computed tomography was performed for fracture typing and evaluation. The mode of injury, time from injury to surgery, time for fracture union, range of motion of the knee, and complications were recorded. Knee joint function was evaluated using the Hospital for Special Surgery Mayo Score (HSS). Results: The mean follow-up period was 21.5 (range, 12-36) months. Fracture united in all patients and the average clinical healing time for fractures was 11.2 ± 1.9 (range, 8-16) weeks. The mean time from injury to surgery was 3.1 ± 1.8 (range, 1-10) days. The mean range of flexion was 131.6° ± 12.5° (range, 110°-145°). The mean range of extension was 1.4°-4.2° (range, -5°-10°). The mean HSS at the final follow-up was 93.5 ± 5.4 (range, 79-100). None of the patients exhibited symptoms of common peroneal nerve injury, knee instability, or upper tibiofibular joint injury. One patient had a superficial infection and was treated with surgical dressing. Conclusion: The partial fibular head osteotomy approach is a feasible alternative for treating posterolateral tibial plateau fractures.

Ozone Decomposition below Room Temperature Using Mn-based Mullite YMn2O5.

A super-low-temperature ozone decomposition is realized without energy consumption on a ternary oxide catalyst mullite YMn2O5 for the first time. The YMn2O5 oxide catalyzed ozone decomposition from a low temperature of -40 °C with 29% conversion (reaction rate: 1534.2 µmol g-1 h-1) and quickly reached 100% (5459.5 µmol g-1 h-1) when warmed up to -5 °C. The superior low-temperature performance over YMn2O5 could surpass that of the reported ozone decomposition catalysts. The structure and element valence characterizations confirmed that YMn2O5 remained the same after 100 h of room-temperature reaction, indicating excellent durability of the catalyst. O2-TPD (O2-temperature-programmed desorption) showed that the active sites are the Mn3+ sites bonded with singly coordinated oxygen on the surface. Combined with in situ Raman measurements and density functional theory calculations, we found that the ozone decomposition reaction on YMn2O5 showed a barrier of only 0.29 eV, following the Eley-Rideal (E-R) mechanism with a rate-limiting step of intermediate O22- desorption. The low barrier minimizes the accumulation of intermediate products and realizes the fast O3 decomposition even at super-low temperatures. Fundamentally, the moderate Mn-O bonding strength in the low-symmetry ternary oxides is crucial to produce singly coordinated active species on the surface responsible for the efficient ozone degradation at low temperatures.

Machine learning and theoretical analysis release the non-linear relationship among ozone, secondary organic aerosol and volatile organic compounds.

Fine particulate matter (PM2.5) and ozone (O3) pollutions are prevalent air quality issues in China. Volatile organic compounds (VOCs) have significant impact on the formation of O3 and secondary organic aerosols (SOA) contributing PM2.5. Herein, we investigated 54 VOCs, O3 and SOA in Tianjin from June 2017 to May 2019 to explore the non-linear relationship among O3, SOA and VOCs. The monthly patterns of VOCs and SOA concentrations were characterized by peak values during October to March and reached a minimum from April to September, but the observed O3 was exactly the opposite. Machine learning methods resolved the importance of individual VOCs on O3 and SOA that alkenes (mainly ethylene, propylene, and isoprene) have the highest importance to O3 formation; alkanes (Cn, n ≥ 6) and aromatics were the main source of SOA formation. Machine learning methods revealed and emphasized the importance of photochemical consumptions of VOCs to O3 and SOA formation. Ozone formation potential (OFP) and secondary organic aerosol formation potential (SOAFP) calculated by consumed VOCs quantitatively indicated that more than 80% of the consumed VOCs were alkenes which dominated the O3 formation, and the importance of consumed aromatics and alkenes to SOAFP were 40.84% and 56.65%, respectively. Therein, isoprene contributed the most to OFP at 41.45% regardless of the season, while aromatics (58.27%) contributed the most to SOAFP in winter. Collectively, our findings can provide scientific evidence on policymaking for VOCs controls on seasonal scales to achieve effective reduction in both SOA and O3.

ZNF703 promotes triple-negative breast cancer cells through cell-cycle signaling and associated with poor prognosis.

BACKGROUND: The oncogenic drivers of triple-negative breast cancer (TNBC), which is characterized by worst prognosis compared with other subtypes, are poorly understood. Although next-generation sequencing technology has facilitated identifying potential targets, few of the findings have been translated into daily clinical practice. The present study is aimed to explore ZNF703 (Zinc finger 703) function and its underlying mechanism in TNBC. METHODS: ZNF703 expressions in tissue microarray were retrospectively examined by immunohistochemistry. The cell proliferation by SRB assay and colony formation assay, as well as cell cycle distribution by flow cytometry were assessed. The protein levels associated with possible underlying molecular mechanisms were evaluated by western blotting. Kaplan-Meier analysis was used to plot survival analysis. RESULTS: Our data suggest that ZNF703 expressed in 34.2% of triple-negative human breast tumors by immunohistochemistry. In vitro, ZNF703 knockdown had potent inhibitory effects on TNBC cell proliferation and cell cycle, with cyclin D1, CDK4, CDK6, and E2F1 downregulated, while Rb1 upregulated. Moreover, Kaplan-Meier analysis showed that high mRNA expression of ZNF703 was correlated to worse overall survival (HR for high expression was 3.04; 95% CI, 1.22 to 7.57, P = 0.017). CONCLUSIONS: Taken together, the results identified that targeting ZNF703 contributed to the anti-proliferative effects in TNBC cells, due to induced G1-phase arrest. This study is the first to identify ZNF703 as a potentially important protein that is involved in TNBC progression.